Molecular and functional characterization of mitochondrial manganese superoxide dismutase from Macrobrachium rosenbergii during bacterial infection. Issue 118 (November 2021)

• Record Type: Journal Article
• Authors: Li, Yanan; Zhan, Fanbin; Li, Fenglin; Lu, Zhijie; Xu, Zizheng; Yang, Youcheng; Shi, Fei; Zhao, Lijuan; Qin, Zhendong; Lin, Li
• Is Part Of: Fish & shellfish immunology. Issue 118(2021)
• Page Start: 94
• Page End: 101
• Publication Date: 2021-11
• Subjects: M. rosenbergii -- MnSOD -- Innate immunity -- Oxidative stress -- Antibacterial immunity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.08.025
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Superoxide dismutases (SODs) are the main antioxidant enzymes involved in alleviating oxidative stress. Although mitochondrial manganese SOD (mMnSOD) has been reported to be correlated with the immune response in crustaceans, its biological properties and role in the immune response remain unclear. Here, we cloned the Macrobrachium rosenbergii mMnSOD ( Mr mMnSOD), analyzed its activity and expression pattern under Staphylococcus aureus and Vibrio parahaemolyticus infection, and further explored its possible mechanism during antibacterial immune response. The results showed that both enzyme activity and the expression of Mr mMnSOD were significantly up-regulated by bacterial infection . Mr mMnSOD knockdown made the prawn susceptible to Vibrio infection, which increased the mortality rate and the number of bacteria in haemocytes. The bacterial agglutination assay confirmed that Mr mMnSOD decreases bacterial abundance via agglutination. Overall, this work identified antibacterial function of Mr mMnSOD in the immune response. In addition to contributing to immunological theory, these findings aid disease prevention and control in crustacean aquaculture. Highlights: mMnSOD widely existed in all tested tissues in healthy M. rosenbergii. The bacterial infection significantly up-regulated the expression of Mr mMnSOD. Mr mMnSOD knockdown made M. rosenbergii significantly sensitive to vibriosis. Mr mMnSOD exerted immune function via bacterial agglutination.