YBX has functional roles in CpG-ODN against cold stress and bacterial infection of Misgurnus anguillicaudatus. Issue 118 (November 2021)

• Record Type: Journal Article
• Authors: Zhao, Zengjue; He, Rongxiao; Chu, Pinpin; Cai, Haiming; Shen, Haokun; Zhao, Zitong; Feng, Saixiang; Cao, Ding; Liao, Ming; Gan, Guanhua; Ye, Hejia; Chen, Zhiyang; Qiu, Weihong; Deng, Jinbo; Ming, Feiping; Ma, Miaopeng; Jia, Junhao; Wu, Jiahui; Huang, Haobin; Sun, Chongjun; Li, Jiayi; Zhang, Linghua
• Is Part Of: Fish & shellfish immunology. Issue 118(2021)
• Page Start: 72
• Page End: 84
• Publication Date: 2021-11
• Subjects: Cold stress -- CpG-ODN -- Y box binding protein -- Misgurnus anguillicaudatus; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.08.018
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Misgurnus anguillicaudatus ( M. anguillicaudatus ) is a widely cultivated fish. However, in M. anguillicaudatus breeding, the frequent cold stress during daily breeding could induce immune suppression and increase the risk of infection, causing serious economic loss. Based on existing findings, CpG Oligonucleotides (CpG-ODNs) may be an ideal protective agent for low temperature fish breeding, performing anti-infective when faced with cold stress with cold shock proteins Y box binding proteins (YBX). Although YBX has pleiotropic functions, its roles in CpG-ODNs-mediated immunity (especially under cold situations) remain largely unexplored. To clarify the relationship among them, we identified the YBX1/YBX2 in M. anguillicaudatus and analyzed using a series of bioinformatics methods. After that, we immunized the fish with 3 types of CpG-ODNs and challenged with Aeromonas hydrophila ( A. hydrophila ). Here we showed that the best anti-bacterial effect of CpG-B was accompanied by the significant upregulation of YBX1. And the detection of the YBX1 downstream effectors confirmed that CpG-B induced the YBX1-mediated Th1 oriented responses to A. hydrophila by regulation of the NLRP3 (Caspase-A/-B), IL-1β, IL-12 and IFN-γ. Afterwards, we found that under cold stress, CpG-B can activate the NLRP3 and NF-κB pathways through YBX1, a key mediator of anti- A. hydrophila in CpG-B immunization. In this study, we demonstrated CpG-B protection against infection in low temperature, and its interaction with YBX1, expanded the research of CpG-ODN under cold stress, and provided a new CpG-ODN application for low temperature fish farming. Highlights: YBX1/YBX2 genes from M. anguillicaudatus were identified and cloned. The protective effects of CpG-ODNs under low temperature were investigated. CpG-B induces a YBX1-mediated Th1 immune response effective even under coldness. Strong YBX1 expression occurs with A. hydrophila infection, CpG-ODNs and cold stress. YBX1 acts as a key mediator in the CpG-B immunization (anti-infection and cold stress responses).