Transcriptional effects of polyethylene microplastics ingestion in developing zebrafish (Danio rerio). (December 2018)

• Record Type: Journal Article
• Authors: LeMoine, Christophe M.R.; Kelleher, Bailey M.; Lagarde, Raphaël; Northam, Caleb; Elebute, Oluwadara O.; Cassone, Bryan J.
• Is Part Of: Environmental pollution. Volume 243(2018)Part A
• Page Start: 591
• Page End: 600
• Publication Date: 2018-12
• Subjects: Transcriptomics -- Respirometry -- Development -- Metabolism -- RNAseq; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2018.08.084
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Over the last few decades, plastic waste has become an increasing environmental concern as it accumulates in every environment on our planet. Though traditionally seen as a macroscopic problem (i.e., large plastic debris), plastic pollution is also evident at smaller scales. Indeed, the intentional industrial production of small plastic particles and the physical degradation of larger plastic debris have overtime resulted in an increased environmental prevalence of smaller plastic particles, including microplastics. While the effects of these small polymers on marine biota have been an important research focus, recent global surveys indicate that our freshwater lakes and rivers are also plagued by microplastics. However, despite these discoveries we currently have a limited understanding of the impact these particles may have on freshwater animals, particularly on vertebrate species. Thus, the aim of the present study was to assess the impact of high concentrations of microplastics (5 and 20 mg.L −1 ) on the early life stages in zebrafish, a model freshwater vertebrate model. To do this, we exposed embryonic and larval zebrafish to fluorescently labelled polyethylene microspheres for up to 14 days and assessed their microplastic content, growth, hatching and oxygen consumption rates. We then explored the molecular underpinnings of the microplastic response by RNA sequencing. Over the course of the exposure, we observed a consistent accumulation of microplastics in the gastrointestinal tract of the fish in a concentration dependent manner, but could not detect any detrimental effects of these particles on larval development, growth or metabolism. However, whole animal transcriptomics revealed that microplastics induced a transient and extensive change in larval gene expression within 48 h exposure, which largely disappeared by 14 days. However, as these transcriptional changes occurred during a critical period of larval development, we suggest that an evaluation of the potential long-term impact of these particles is warranted. Graphical abstract: Image 1 Highlights: No effects of MP (microplastics) on hatching, mortality and growth in zebrafish No effects of MP on O2 consumption rates Large transcriptomic changes after 2 days of MP exposure Downregulated genes involved with neural development and function Most gene expression differences disappeared at 14 days of MP exposure Abstract : Short term exposure of developing zebrafish to polyethylene microplastics induces extensive transcriptomic perturbations with no apparent immediate effects on larval development and physiology.