Desmiflavasides A and B: Two new bioactive pregnane glycosides from the sap of Desmidorchis flava. (June 2015)

• Record Type: Journal Article
• Authors: Raees, Muhammad Adil; Hussain, Hidayat; Rehman, Najeeb Ur; Khan, Husain Yar; Abbas, Ghulam; Al-Rawahi, Ahmed; Elyassi, Ali; Al-Amri, Issa Sulaiman; Green, Ivan R.; Al-Broumi, Mohammed Abdullah; Mahmood, Talat; Al-Harrasi, Ahmed
• Is Part Of: Phytochemistry letters. Volume 12(2015:Jun.)
• Page Start: 153
• Page End: 157
• Publication Date: 2015-06
• Subjects: Desmidorchis flava -- NMR -- Pregnane glycoside -- Anticancer activity; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2015.03.013
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: Two new metabolites named desmiflavasides A and B were isolated from Desmidorchis flava . Two new structures were elucidated by 1D NMR, 2D NMR, and ESIMS experiments. Desmiflavaside B induced a 27.4% (conc. 75 μg/ml) and 33.1% (conc. 100 μg/ml) growth inhibition of breast cancer cell. Desmiflavasides A and B showed weak antioxidant and urease enzyme inhibition. Abstract: The sap from the succulent, Desmidorchis flava (N.E.Br) Meve & Liede (Apocyanaceae), provided two new pregnane glycosides named desmiflavasides A (1 ) and B (2 ) whose structures were established from 1D and 2D NMR spectroscopic techniques and mass spectromentry (ESIMS) measurements. Desmiflavaside B (2 ) was tested for anticancer activity and induced a 27.4% and 33.1% growth inhibition of the breast cancer cell line (MDA MB231) at a concentration of 75 μg/ml and 100 μg/ml, respectively. Desmiflavasides A (1 ) and B (2 ) were additionally evaluated for: DPPH antioxidant activity, urease enzyme inhibition as well as for xanthine oxidase enzyme, α-glucosidase enzyme and acetylcholinesterase inhibition activities. They displayed weak antioxidant and urease enzyme inhibition activities with 15% inhibition.