Gene cloning, expression and immune adjuvant properties of the recombinant fusion peptide Tα1-BLP on avian influenza inactivate virus vaccine. (July 2018)

• Record Type: Journal Article
• Authors: Zhang, Cong; Zhou, Jiangfei; Cai, Kairui; Zhang, Wufan; Liao, Chengshui; Wang, Chen
• Is Part Of: Microbial pathogenesis. Volume 120(2018)
• Page Start: 147
• Page End: 154
• Publication Date: 2018-07
• Subjects: Thymosin α1 (Tα1) -- Bursin-like peptide (BLP) -- Fusion peptide -- Avian influenza vaccine -- Adjuvant; Tα1 Thymosin α1 -- BLP Bursin-like peptide -- Tα1-BLP thymosin α1-bursin-like peptide -- AIV Avian influenza virus -- BS Bursin -- CTL Cytotoxic T-lymphocyte -- IFN-γ Interferon-γ -- IL-4 Th2-type cytokines -- HA hemagglutination -- Th T helper type -- Th1 T helper type 1 -- Th2 T helper type 2 -- WIV whole-inactivated virus -- NA neuraminidase; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.05.003
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Thymosin α1 (Tα1) and bursin-like peptide (BLP) are both immunopotentiators. In order to investigate adjuvant of thymosin α1-bursin-like peptide (Tα1-BLP), we cloned the gene of Tα1-BLP and provided evidence that the gene of Tα1-BLP in a recombinant prokaryotic expression plasmid was successfully expressed in E. coli BL21. To evaluate the immune adjuvant properties of Tα1-BLP, chickens were immunized with Tα1-BLP combined with H9N2 avian influenza whole-inactivated virus (WIV). The titers of HI antibody, antigen-specific antibodies, AIV-neutralizing antibodies, levels of Th1-type cytokines (IFN-γ) and Th2-type cytokines (IL-4) and lymphocyte proliferation responses were determined. What's more, the viral loads and pathologic changes of lung tissue were observed by virus challenge experiment and HE staining to evaluate the immune protection of chickens. We found that Tα1-BLP enhanced HI antibody and antigen-specific IgG antibodies titers, increased the level of AIV-neutralizing antibodies, induced the secretion of Th1- and Th2-type cytokines, and promoted the proliferation of T and B lymphocyte, Furthermore, virus challenge experiment and HE staining confirmed that Tα1-BLP contributed to inhibition replication of the virus from chicken lungs and protected the lungs from damage. Altogether, this study suggested that Tα1-BLP is a novel adjuvant suitable for H9N2 avian influenza vaccine. Highlights: The gene of Tα1-BLP in a recombinant prokaryotic expression plasmid was successfully expressed in E. coli BL21. Tα1-BLP able to stimulate specific immune responses in chicken with H9N2 avian influenza inactivated virus as antigen. Tα1-BLP is a novel adjuvant suitable for H9N2 avian influenza vaccine.