Optimization of limonene biotransformation for the production of bulk amounts of α-terpineol. (December 2019)

• Record Type: Journal Article
• Authors: Molina, Gustavo; Pessôa, Marina G.; Bicas, Juliano L.; Fontanille, Pierre; Larroche, Christian; Pastore, Gláucia M.
• Is Part Of: Bioresource technology. Volume 294(2019)
• Publication Date: 2019-12
• Subjects: Aroma -- Bioflavors -- Limonene -- Sphingobium sp. -- Response surface methodology; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122180
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Bioprocess for α-terpineol production, a commercially important monoterpene alcohol. Effect of medium composition and cultivation conditions in α-terpineol production. Optimization process for the bioproduction of α-terpineol from limonene. Advances in the bioprocess using biphasic system for limonene bioconversion. One of the highest production of the bioaroma α-terpineol already reported. Abstract: The biotransformation of R -(+)-limonene into high concentrations of R -(+)-α-terpineol by Sphingobium sp. was investigated in order to optimize the main process variables (pH, biocatalyst concentration, substrate concentration, temperature and agitation). This strategy comprised the screening of variables by a Plackett-Burman design followed by a Central Composite Design. The statistical analysis showed that the optimal α-terpineol production were at 28 °C and pH 7.0, with a limonene concentration of 350 g/L of organic phase agitation of 200 rpm and a biocatalyst concentration of 2.8 g/L of aqueous phase (OD600 = 8). Further trials showed that the R -(+)-α-terpineol concentration was higher (240 g/L after 96 h) when using a ratio of 1:3 (v.v −1 ) of organic:aqueous phases. However, the total production and yield (in terms of biomass) of α-terpineol would be maximized for an aqueous:organic ratio of 1:1. The experimental design optimization adopted herein was an effective tool for this type of study.