Antimicrobial activity of nanoemulsion on drug-resistant bacterial pathogens. (July 2018)

• Record Type: Journal Article
• Authors: Krishnamoorthy, Rajapandiyan; Athinarayanan, Jegan; Periasamy, Vaiyapuri Subbarayan; Adisa, Abdulraheem R.; Al-Shuniaber, Mohammed A.; Gassem, Mustafa A.; Alshatwi, Ali A.
• Is Part Of: Microbial pathogenesis. Volume 120(2018)
• Page Start: 85
• Page End: 96
• Publication Date: 2018-07
• Subjects: Nanoemulsion -- Drug-resistant bacteria -- Extended-spectrum beta-lactamase -- Methicillin-resistant Staphylococcus aureus -- Drug efflux pump inhibitor; AB antibiotics -- ATCC American Type Culture Collection strains -- DR drug-resistant -- DRSP drug-resistant Streptococcus pyogenes -- EC Escherichia coli -- EPI efflux pump inhibitor -- ESBL extended-spectrum beta-lactamase -- KP Klebsiella pneumonia -- MBC minimum bactericidal concentration -- MIC minimum inhibitory concentration -- MRSA methicillin-resistant Staphylococcus aureus -- PA Pseudomonas aeruginosa -- SA Staphylococcus aureus.; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.04.035
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The appearance of drug-resistant (DR) bacteria in the community is a crucial development, and is associated with increased morbidity, mortality, healthcare costs, and antibiotic use. Natural oil nanoemulsions (NEs) have potential for antimicrobial applications. In the present study, we determined the antimicrobial activity of an NE against DR bacterial pathogens in vitro . The NE comprised Cleome viscosa essential oil, Tween 80 nonionic surfactant, and water. We found that an NE with a droplet size of 7 nm and an oil:surfactant (v/v) ratio of 1:3 was effective against methicillin-resistant Staphylococcus aureus (MRSA), DR Streptococcus pyogenes, and DR extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa . Fourier-transform infrared (FTIR) spectroscopy revealed that NE treatment modified the functional groups of lipids, proteins, and nucleic acids in DR bacterial cells. Scanning electron microscopy (SEM) showed damage to the cell membranes and walls of NE-treated DR bacteria. These alterations were caused by bioactive compounds with wide-spectrum enzyme-inhibiting activity in the NE, such as β-sitosterol, demecolcine, campesterol, and heneicosyl formate. The results suggest that the nanoemulsion is effective against DR bacteria, and acts by inhibiting the drug efflux mechanism of DR strains. Highlights: Cleome viscosa essential oil nanoemulsion (NE) is an efficient antimicrobial agent against drug resistance bacterial pathogens. NE exhibited bactericidal activity against MRSA and ESBL producer and inhibiting the bacterial efflux pumps mechanisms. NE showed broad spectrum bactericidal activities by altering their functional groups in the cell wall, lipids, proteins and nucleic acid in both drug resistant Gram positive and Gram negative bacterial pathogens.