PH-responsive lignin-based magnetic nanoparticles for recovery of cellulase. (December 2019)

• Record Type: Journal Article
• Authors: Dong, Rui-jing; Zheng, Da-feng; Yang, Dong-jie; Qiu, Xue-qing
• Is Part Of: Bioresource technology. Volume 294(2019)
• Publication Date: 2019-12
• Subjects: Lignin -- pH-responsive magnetic nanoparticle -- Cellulase immobilization -- Desorption -- Recovery of cellulase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122133
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: pH-responsiveness Fe3 O4 /LSQA recovery of cellulase through adjusting the pH. The immobilization ratio of cellulase was 55.52% at pH 4.8. The desorption ratio was 68.27% at pH 6.8. After 5 recycles, the desorbed cellulase retained 31.79% relative activity. Abstract: Enzymatic hydrolysis of lignocellulose to produce bioethanol by cellulase is an important method to alleviate the energy crisis. In this paper, in order to overcome the shortcomings of low efficiency, high cost and easy deactivation of cellulase in the process of bio-refinery, pH-responsive lignin-based magnetic nanoparticles (Fe3 O4 /LSQA) were synthesized to immobilize and recover cellulase. It was shown that a high immobilization ratio of 55.52% for cellulase was obtained. Meanwhile, the desorption ratio was 68.27% by adjusting the pH of the system. After five reusing cycles, the desorbed cellulase retained 31.79% of the relative activity due to the pH responsiveness of Fe3 O4 /LSQA. These results not only provide a new idea for the recycling of cellulase, but also broaden the application of industrial lignin and increase the extra value.