Carbon selection for nitrogen degradation pathway by Stenotrophomonas maltophilia: Based on the balances of nitrogen, carbon and electron. (December 2019)

• Record Type: Journal Article
• Authors: Jia, Yating; Zhou, Miaomiao; Chen, Yuancai; Luo, Jun; Hu, Yongyou
• Is Part Of: Bioresource technology. Volume 294(2019)
• Publication Date: 2019-12
• Subjects: Carbon selection -- Assimilation -- Heterotrophic nitrification–aerobic denitrification (HN-AD) -- Mass balance -- Electron distribution; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122114
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: S. mailophilia DQ01 had a rapid growth and a large specific removal rate ( μmax ) Inorganic nitrogen was mainly assimilated by HN-AD bacterium S. mailophilia DQ01. The carbon selection for nitrogen removal pathway occurred in S. mailophilia. Carbohydrate was beneficial to HN-AD due to the alkalization and high reduction. Carbon sources, C/N and reaction driver force could regulate the electron transfer. Abstract: A novel strain DQ01 capable of simultaneous removal of nitrate and ammonium under the aerobic condition was isolated from the landfill leachate and identified as S tenotrophomonas maltophilia . The result showed that S. maltophilia had carbon selection for the nitrogen removal pathway, and preferred to utilize carboxylate rather than carbohydrate, as carboxylate could directly participate in TCA cycle without Embden Meyerhof Parmas (EMP). Nitrogen and carbon balances confirmed that the ammonium assimilation was the main or even sole removal pathway for S. maltophilia, and carboxylate was more conducive to heterotrophic nitrification-aerobic denitrification (HN-AD) process due to the serious self-alkalization and higher reduction potential of carboxylate, which followed: NH4 + → NO2 − → NO3 − → NO2 − → NO due to the lack of nor and nos . Meanwhile, the higher C/N and nitrate could generate a more powerful ion transport driving force to accelerate the electron transfer in the denitrifying respiratory chain.