Assessment of MR‐based R2* and quantitative susceptibility mapping for the quantification of liver iron concentration in a mouse model at 7T. Issue 5 (25th March 2018)

• Record Type: Journal Article
• Authors: Simchick, Gregory; Liu, Zhi; Nagy, Tamas; Xiong, May; Zhao, Qun
• Is Part Of: Magnetic resonance in medicine. Volume 80:Issue 5(2018)
• Page Start: 2081
• Page End: 2093
• Publication Date: 2018-03-25
• Subjects: liver iron concentration -- quantitative susceptibility mapping -- R2* mapping -- liver iron overload -- mouse model; Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.27173
• Languages: English
• ISSNs: 0740-3194

Abstract

Abstract : Purpose: To assess the feasibility of quantifying liver iron concentration (LIC) using R 2 * and quantitative susceptibility mapping (QSM) at a high field strength of 7 Tesla (T). Methods: Five different concentrations of Fe‐dextran were injected into 12 mice to produce various degrees of liver iron overload. After mice were sacrificed, blood and liver samples were harvested. Ferritin enzyme‐linked immunosorbent assay (ELISA) and inductively coupled plasma mass spectrometry were performed to quantify serum ferritin concentration and LIC. Multiecho gradient echo MRI was conducted to estimate R 2 * and the magnetic susceptibility of each liver sample through complex nonlinear least squares fitting and a morphology enabled dipole inversion method, respectively. Results: Average estimates of serum ferritin concentration, LIC, R 2 *, and susceptibility all show good linear correlations with injected Fe‐dextran concentration; however, the standard deviations in the estimates of R 2 * and susceptibility increase with injected Fe‐dextran concentration. Both R 2 * and susceptibility measurements also show good linear correlations with LIC (R 2 = 0.78 and R 2 = 0.91, respectively), and a susceptibility‐to‐LIC conversion factor of 0.829 ppm/(mg/g wet) is derived. Conclusion: The feasibility of quantifying LIC using MR‐based R 2 * and QSM at a high field strength of 7T is demonstrated. Susceptibility quantification, which is an intrinsic property of tissues and benefits from being field‐strength independent, is more robust than R 2 * quantification in this ex vivo study. A susceptibility‐to‐LIC conversion factor is presented that agrees relatively well with previously published QSM derived results obtained at 1.5T and 3T.