Comparison of influenza virus replication fidelity in vitro using selection pressure with monoclonal antibodies. Issue 6 (15th April 2013)

• Record Type: Journal Article
• Authors: Wong, Karen Ka Yin; Rockman, Steve; Ong, Chi; Bull, Rowena; Stelzer‐Braid, Sacha; Rawlinson, William
• Is Part Of: Journal of medical virology. Volume 85:Issue 6(2013:Jun.)
• Page Start: 1090
• Page End: 1094
• Publication Date: 2013-04-15
• Subjects: influenza -- replication fidelity -- polymerase -- monoclonal antibodies -- hemagglutinin; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.23532
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract: The replication fidelity of reassortant A/Fujian/411/02(H3N2)‐like influenza viruses was assessed by in vitro detection of escape mutants developing under selective pressure from monoclonal antibodies. The results showed A/Wyoming/3/03(H3N2) possessed lower fidelity relative to the A/California/7/04(H3N2) and A/Wisconsin/57/05(H3N2) viruses through the emergence of mutant viruses carrying H156Q hemagglutinin mutation which allows antibody escape. Using the neutralization assay to compare the fidelity of reassortant pandemic A/California/7/09(H1N1) viruses, the pandemic virus was shown to possess relative higher fidelity compared to A/Wyoming/3/03. This higher fidelity may contribute to the lack of major antigenic changes in the pandemic virus since the emergence in 2009. J. Med. Virol. 85: 1090–1094, 2013. © 2013 Wiley Periodicals, Inc.