In Vivo Evidence for a Prodrug Activation Mechanism during Colibactin Maturation. Issue 10 (6th June 2013)

• Record Type: Journal Article
• Authors: Bian, Xiaoying; Fu, Jun; Plaza, Alberto; Herrmann, Jennifer; Pistorius, Dominik; Stewart, A. Francis; Zhang, Youming; Müller, Rolf
• Is Part Of: Chembiochem. Volume 14:Issue 10(2013)
• Page Start: 1194
• Page End: 1197
• Publication Date: 2013-06-06
• Subjects: biosynthesis -- colibactin -- E. coli Nissle -- gene expression -- prodrug maturation; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300208
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : Releasing the cytopath: We have identified an N ‐myristoyl‐D ‐asparagine (1 ) as the free N‐terminal prodrug scaffold in cytopathogenic Escherichia coli strains expressing the colibactin gene cluster. Colibactin is released in vivo upon cleavage of precolibactin. We provide for the first time in vivo evidence of the prodrug‐like release mechanism of colibactin.