A 3D cell culture system: Separation distance between INS‐1 cell and endothelial cell monolayers co‐cultured in fibrin influences INS‐1 cells insulin secretion. Issue 2 (24th September 2012)

• Record Type: Journal Article
• Authors: Sabra, Georges; Vermette, Patrick
• Is Part Of: Biotechnology and bioengineering. Volume 110:Issue 2(2013:Feb.)
• Page Start: 619
• Page End: 627
• Publication Date: 2012-09-24
• Subjects: beta cells -- INS‐1 cells -- HUVEC -- 3D cell culture -- diabetes -- insulin secretion; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.24716
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: The aim of this study was to develop an in vitro cell culture system allowing studying the effect of separation distance between monolayers of rat insulinoma cells (INS‐1) and human umbilical vein endothelial cells (HUVEC) co‐cultured in fibrin over INS‐1 cell insulin secretion. For this purpose, a three‐dimensional (3D) cell culture chamber was designed, built using micro‐fabrication techniques and validated. The co‐culture was successfully carried out and the effect on INS‐1 cell insulin secretion was investigated. After 48 and 72 h, INS‐1 cells co‐cultured with HUVEC separated by a distance of 100 µm revealed enhanced insulin secretion compared to INS‐1 cells cultured alone or co‐cultured with HUVEC monolayers separated by a distance of 200 µm. These results illustrate the importance of the separation distance between two cell niches for cell culture design and the possibility to further enhance the endocrine function of beta cells when this factor is considered. Biotechnol. Bioeng. 2013; 110: 619–627. © 2012 Wiley Periodicals, Inc. Abstract : A three‐dimensional in vitro cell culture chamber was designed, built using micro‐fabrication techniques and validated. This cell culture system allowed study of the effect of separation distance between monolayers of rat insulinoma cells (INS‐1) and human umbilical vein endothelial cells (HUVEC) co‐cultured in fibrin over INS‐1 cells insulin secretion. Sabra and Vermette report the importance of the separation distance between two cell niches for cell culture design and the possibility to enhance beta cells function.