In vitro biomineralization on poly(vinyl alcohol)/biphasic calcium phosphate hydrogels. Issue 2 (14th January 2020)

• Record Type: Journal Article
• Authors: Nie, Lei; Li, Xingchen; Wang, Zheng; Hu, Kehui; Cai, Ruihua; Li, Pei; Han, Yanting; Sun, Meng; Yuan, Hongyu; Suo, Jinping; Yang, Shoufeng
• Is Part Of: Bioinspired, biomimetic and nanobiomaterials. Volume 9:Issue 2(2020)
• Page Start: 122
• Page End: 128
• Publication Date: 2020-01-14
• Subjects: apatite -- mineralisation -- nanocomposites; Biomimetic materials -- Periodicals; Nanobiotechnology -- Periodicals; Biotechnology -- Periodicals; 660.605
• DOI: 10.1680/jbibn.19.00051
• Languages: English
• ISSNs: 2045-9858

Abstract

Abstract : Biomineralized tissue is considered the final product of successful cell culture in bone tissue engineering. Dulbecco's modified Eagle's medium (DMEM) with fetal bovine serum (FBS) not only is used as a common culture medium but also provides a natural biomineralization environment, due to having similar ionic concentrations as blood plasma. Here, poly(vinyl alcohol) hydrogel with incorporated biphasic calcium phosphate nanoparticles was immersed in a DMEM–FBS cell culture medium, and then biomineralization occurred on the nanocomposite surface, which was characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. Such formed bone-like apatite on the surface facilitated the proliferation of osteoblasts, identified by Cell Counting Kit-8 analysis and fluorescent microscopy. This study verified the spontaneous biomineralization on the surface of a calcium phosphate-based nanocomposite by using a simple DMEM–FBS immersion strategy, which was promising for biomodification of bone substitutes.