Robertkochia solimangrovi sp. nov., isolated from mangrove soil, and emended description of the genus Robertkochia. Issue 3 (24th March 2020)

• Record Type: Journal Article
• Authors: Lam, Ming Quan; Vodovnik, Maša; Zorec, Maša; Chen, Sye Jinn; Goh, Kian Mau; Yahya, Adibah; Md Salleh, Madihah; Ibrahim, Zaharah; Tokiman, Lili; McQueen-Mason, Simon J.; Bruce, Neil C.; Chong, Chun Shiong
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 3(2020)
• Page Start: 1769
• Page End: 1776
• Publication Date: 2020-03-24
• Subjects: Robertkochia solimangrovi -- polyphasic taxonomy -- Flavobacteriaceae -- mangrove; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003970
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : To date, there is sparse information for the genus Robertkochia with Robertkochia marina CC-AMO-30D T as the only described member. We report here a new species isolated from mangrove soil collected at Malaysia Tanjung Piai National Park and perform polyphasic characterization to determine its taxonomic position. Strain CL23 T is a Gram-negative, yellow-pigmented, strictly aerobic, catalase-positive and oxidase-positive bacterium. The optimal growth conditions were determined to be at pH 7.0, 30–37 °C and in 1–2 % (w/v) NaCl. The major respiratory quinone was menaquinone-6 (MK-6) and the highly abundant polar lipids were four unidentified lipids, a phosphatidylethanolamine and two unidentified aminolipids. The 16S rRNA gene similarity between strain CL23 T and R. marina CC-AMO-30D T is 96.67 %. Strain CL23 T and R. marina CC-AMO-30D T clustered together and were distinguished from taxa of closely related genera in 16S rRNA gene phylogenetic analysis. Genome sequencing revealed that strain CL23 T has a genome size of 4.4 Mbp and a G+C content of 40.72 mol%. Overall genome related indexes including digital DNA–DNA hybridization value and average nucleotide identity are 17.70 % and approximately 70%, below the cutoffs of 70 and 95%, respectively, indicated that strain CL23 T is a distinct species from R. marina CC-AMO-30D T . Collectively, based on the phenotypic, chemotaxonomic, phylogenetic and genomic evidences presented here, strain CL23 T is proposed to represent a new species with the name Robertkochia solimangrovi sp. nov. (KCTC 72252 T =LMG 31418 T ). An emended description of the genus Robertkochia is also proposed.