Penicillin susceptibility among invasive MSSA infections: a multicentre study in 16 Spanish hospitals. (10th July 2021)

• Record Type: Journal Article
• Authors: Mama, Olouwafemi Mistourath; Aspiroz, Carmen; Lozano, Carmen; Ruiz-Ripa, Laura; Azcona, José Manuel; Seral, Cristina; Cercenado, Emilia; López-Cerero, Lorena; Palacian, Pilar; Belles-Belles, Alba; Berdonces, Pilar; Siller, María; Aguirre-Quiñonero, Amaia; Zarazaga, Myriam; Torres, Carmen
• Is Part Of: Journal of antimicrobial chemotherapy. Volume 76:Number 10(2021)
• Page Start: 2519
• Page End: 2527
• Publication Date: 2021-07-10
• Subjects: Anti-infective agents -- Periodicals; Chemotherapy -- Periodicals; 615.58
• DOI: 10.1093/jac/dkab208
• Languages: English
• ISSNs: 0305-7453

Abstract

Abstract: Objectives: To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PEN S ) isolates. Methods: A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018–19 (6–12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PEN S isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF / lukS-PV, eta, etb and tst . The immune evasion cluster (IEC) type was also analysed. All the MSSA-PEN S isolates were submitted to S. aureus protein A ( spa ) typing and the clonal complexes (CCs) were assigned according to their spa type. Results: The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PEN S - blaZ negative . MSSA-PEN S - blaZ negative isolates ( n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PEN S - blaZ negative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PEN S - blaZ negative isolates were IEC negative and four isolates were Panton–Valentine leucocidin ('PVL') positive. Conclusions: A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PEN S clones could be responsible for these data. The detection among MSSA-PEN S - blaZ negative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.