Co-expression analysis identifies networks of miRNAs implicated in biological ageing and modulated by short-term interval training. (October 2021)

• Record Type: Journal Article
• Authors: Kumar Dev, Prasun; Gray, Adrian J.; Scott-Hamilton, John; Hagstrom, Amanda D.; Murphy, Aron; Denham, Joshua
• Is Part Of: Mechanisms of ageing and development. Volume 199(2021)
• Publication Date: 2021-10
• Subjects: T/S telomere to single copy gene -- sncRNA small non-coding RNA -- SIT sprint interval training -- V˙O2peak peak oxygen uptake -- Pmax maximum power output (during incremental exercise testing) -- PTT power output during a 20-min cycling time-trial -- DE differential expression -- FDR false discovery rate; Small non-coding RNA -- Exercise training -- Telomere length -- Gene -- WGCNA; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2021.111552
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Leukocyte telomere length is positively correlated with maximal aerobic fitness. Leukocyte telomere length is correlated to unique co-expressed miRNAs networks. Co-expressed miRNA networks controlled by exercise were identified using WGCNA. Specific miRNA networks are involved in exercise responses and telomere regulation. Abstract: Exercise training seems to promote healthy biological ageing partly by inducing telomere maintenance, yet the molecular mechanisms are not fully understood. Recent studies have emphasised the importance of microRNAs (miRNAs) in ageing and their ability to mirror pathophysiological alterations associated with age-related diseases. We examined the association between aerobic fitness and leukocyte telomere length before determining the influence of vigorous exercise training on the regulation of leukocyte miRNA networks. Telomere length was positively correlated to aerobic fitness ( r = 0.32, p = 0.02). 104 miRNAs were differentially expressed after six weeks of thrice-weekly sprint interval training (SIT) in healthy men ( q < 0.05). Gene co-expression analysis (WGCNA) detected biologically meaningful miRNA networks, five of which were significantly correlated with pre-SIT and post-SIT expression profiles ( p < 0.001) and telomere length. Enrichment analysis revealed that the immune response, T cell differentiation and lipid metabolism associated miRNAs clusters were significantly down-regulated after SIT. Using data acquired from the Gene Expression Omnibus (GEO), we also identified two co-expressed miRNAs families that were modulated by exercise training in previous investigations. Collectively, our findings highlight the miRNA networks implicated in exercise adaptations and telomere regulation, and suggest that SIT may attenuate biological ageing through the control of the let-7 and miR-320 miRNA families.