Regulation of Ovarian Cancer Cell Adhesion and Invasion by Chloride Channels. Issue 4 (1st April 2009)

• Record Type: Journal Article
• Authors: Li, Min; Wang, Qing; Lin, Wei; Wang, Bo
• Is Part Of: International journal of gynecological cancer. Volume 19:Issue 4(2009)
• Page Start: 526-530
• Page End: 526-530
• Publication Date: 2009-04-01
• Subjects: Chloride channels -- Cell adhesion -- Cell invasion -- Ovarian cancer cells; Generative organs, Female -- Cancer -- Periodicals; 616.99465
• DOI: 10.1111/IGC.0b013e3181a3d6d2
• Languages: English
• ISSNs: 1048-891X

Abstract

Abstract : The purpose of this study was to investigate the effects of chloride (Cl − ) channels on the adhesive and invasive potentials of human ovarian cancer cell line A2780. By using the adhesion and Transwell invasion assays, we showed that 4, 4′-diisothiocyanatostilbene-2, 2′-disulfonic acid (200 μmol/L), a nonselective Cl − channel blocker, significantly inhibits cancer cell adhesion and invasion. 5-Nitro-2-(3-phenylpropylamino)-benzoate (200 μmol/L), niflumic acid (100 μmol/L), and tamoxifen (30 μmol/L) had similar inhibitory effects. Regulatory volume decrease is markedly suppressed by 4, 4′-diisothiocyanatostilbene-2, 2′-disulfonic acid, 5-nitro-2-(3-phenylpropylamino)-benzoate, niflumic acid, and tamoxifen. Moreover, intracellular calcium concentration ([Ca 2+ ]i ) measurements indicated that a Cl − channel-mediated increase in [Ca 2+ ]i is also one of the mechanisms of cancer cell adhesion and invasion. Our results strongly suggest that Cl − channels may regulate ovarian cancer cell adhesion and invasion, probably through inducing a regulatory volume decrease and mediating a [Ca 2+ ]i increase.