Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit. Issue 11 (November 2021)

• Record Type: Journal Article
• Authors: Huang, Sheng; Xu, Kangli; Xu, Shanshan; Yu, Shijun; Miao, Yan; Wang, Juan
• Is Part Of: Journal of international medical research. Volume 49:Issue 11(2021)
• Publication Date: 2021-11
• Subjects: Flow cytometry -- CD34 -- stem cell -- cord blood -- stem cell enumeration kit -- BD Biosciences; Medicine -- Periodicals; Pharmacology -- Periodicals; 610.5
• DOI: 10.1177/03000605211055384
• Languages: English
• ISSNs: 0300-0605

Abstract

Objective: To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences. Methods: Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software. Results: A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: −5.7% to−14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: −2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961–0.9972) and 0.9512 (95% CI: 0.9423–0.9587) for the BD Biosciences and Beckman Coulter kits, respectively. Conclusions: The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples.