High-quality RNA improves sensitivity of SARS-CoV-2 detection by colorimetric RT-LAMP. Issue 3 (February 2022)

• Record Type: Journal Article
• Authors: Puigmulé, Marta; Coll, Mònica; Pérez-Serra, Alexandra; López, Laura; Picó, Ferran; Neto, Nuria; Corona, Mònica; Pinsach-Abuin, Mel·lina; Ferrer-Costa, Carles; Buxó, Maria; Queralt, Francesc-Xavier; Brugada, Ramon
• Is Part Of: Experimental biology and medicine. Volume 247:Issue 3(2022)
• Page Start: 276
• Page End: 281
• Publication Date: 2022-02
• Subjects: SARS-CoV-2 -- RT-LAMP -- high-quality RNA; Physiology -- Periodicals; Biology, Experimental -- Periodicals; Medicine, Experimental -- Periodicals; 610.72
• DOI: 10.1177/15353702211054768
• Languages: English
• ISSNs: 1535-3702

Abstract

The global SARS-CoV-2 pandemic requires a rapid, reliable, and user-friendly diagnostic test to help control the spread of the virus. Reverse transcription and quantitative PCR (RT-qPCR) is currently the gold standard method for SARS-CoV-2 detection. Here, we develop a protocol based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and demonstrate increased sensitivity of this technique using fresh RNA extracts compared to RNA samples subjected to freezing/thawing cycles. We further compare RT-LAMP to RT-qPCR and demonstrate that the RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct values between 8 and 35.