Microbubble destruction with ultrasound augments neovascularisation by bone marrow cell transplantation in rat hind limb ischaemia. Issue 4 (1st July 2005)

• Record Type: Journal Article
• Authors: Enomoto, S; Yoshiyama, M; Omura, T; Matsumoto, R; Kusuyama, T; Nishiya, D; Izumi, Y; Akioka, K; Iwao, H; Takeuchi, K; Yoshikawa, J
• Is Part Of: Heart. Volume 92:Issue 4(2006)
• Page Start: 515
• Page End: 520
• Publication Date: 2005-07-01
• Subjects: BM-MNC, bone marrow mononuclear cells -- BMT, bone marrow derived mononuclear cell transplantation -- EPC, endothelial progenitor cell -- LDBF, laser Doppler blood flow -- LDBFI, laser Doppler blood flow index -- MB, microbubble destruction with ultrasound -- PCR, polymerase chain reaction -- VEGF, vascular endothelial growth factor; microbubble -- ultrasound -- bone marrow mononuclear cells -- neovascularisation -- limb ischaemia; Heart -- Diseases -- Treatment -- Periodicals; Cardiology -- Periodicals; 616.12
• DOI: 10.1136/hrt.2005.064162
• Languages: English
• ISSNs: 1355-6037

Abstract

Abstract : Objective: To examine the effects of microbubble destruction with ultrasound (MB) combined with bone marrow derived mononuclear cell transplantation (BMT) into ischaemic tissues in rat hind limb ischaemia. Methods and results: Unilateral hind limb ischaemia was surgically induced in Lewis rats. At postoperative day 7, rats were randomly divided into three groups: a vehicle treated group, an ultrasound treated group, and an MB treated group. MB treatment increased vascular endothelial growth factor mRNA as assessed by real time polymerase chain reaction (3.0-fold, p < 0.05). At four weeks, the MB group had increases in laser Doppler blood flow index (LDBFI; 1.2-fold, p < 0.05), angiographically detectable collateral vessels (angiographic score: 1.4-fold, p < 0.01), and capillary to muscle fibre ratio (1.4-fold, p < 0.01) in ischaemic limbs compared with the vehicle treated group. No differences were seen between the vehicle and ultrasound treated groups. Secondly, rats were allocated to vehicle treatment, BMT (5 × 10 6 cells/rat), or a combination of MB and BMT (MB+BMT) at seven days after hind limb ischaemia. BMT treatment significantly increased LDBFI, angiographic score, and capillary to muscle fibre ratio compared with vehicle treatment. Interestingly, MB+BMT treatment produced significantly greater LDBFI (1.2-fold, p < 0.01), angiographic score (1.5-fold, p < 0.01), and capillary to muscle fibre ratio (1.5-fold, p < 0.05) than BMT treatment alone. Conclusions: MB may be a useful technique to enhance BMT induced neovascularisation.