Antimicrobial and Antibiofilm Activities of 4, 5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp. (27th August 2021)

• Record Type: Journal Article
• Authors: Damim, Andressa C.; de Albuquerque, Danilo Y.; Dantas, Fabiana G. S.; Galvão, Fernanda; Negri, Melyssa; Pizzuti, Lucas; Casagrande, Gleison A.; Saba, Sumbal; Rafique, Jamal; de Oliveira, Kelly M. P.
• Other Names: Azzazy Hassan Academic Editor.
• Is Part Of: Journal of chemistry. Volume 2021(2021)
• Publication Date: 2021-08-27
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1155/2021/5587318
• Languages: English
• ISSNs: 2090-9063

Abstract

Abstract : In the present study, the antimicrobial and antibiofilm activities of two 4, 5-dihydro-1H-pyrazole-1-carboximidamide hydrochloride, (trifluoromethyl) phenyl-substituted (compound 1 ) and bromophenyl-substituted (compound 2 ), were evaluated against four Salmonella spp. serotypes through broth microdilution and biofilm-forming activity. Further, the cytotoxicity of the compounds was evaluated by cell viability assays using cultures of HeLa and Vero cell lines, and the mutagenic potential was assessed by the Ames test. In the broth microdilution test, compound 1 inhibited 90% of the strains tested at the minimum inhibitory concentration of 62.5 μ g mL −1 . Furthermore, both compounds prevented biofilm formation, with a reduction of up to 5.2 log10 . HeLa and Vero cells exhibited 100% viability in the presence of compound 1 . In contrast, low cell viability was observed in the presence of 15 µ g mL −1 of compound 2 . Furthermore, no mutagenic potential was detected at any of the tested concentrations of compound 1 .