Inactivation of airborne bacteria by plasma treatment and ionic wind for indoor air cleaning. Issue 9 (25th May 2020)

• Record Type: Journal Article
• Authors: Prehn, Franziska; Timmermann, Eric; Kettlitz, Manfred; Schaufler, Katharina; Günther, Sebastian; Hahn, Veronika
• Is Part Of: Plasma processes and polymers. Volume 17:Issue 9(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-05-25
• Subjects: cold plasma -- HEPA filter -- hospital‐acquired infections -- HVAC system -- UV irradiation; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.202000027
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract: Airborne bacteria are a general problem in medical or health care facilities with a high risk for nosocomial infections. Rooms with a continuous airflow, such as operation theaters, are of particular importance due to a possible dissemination and circulation of pathogens including multidrug‐resistant microorganisms. In this regard, a cold atmospheric‐pressure plasma (CAP) may be a possibility to support usual disinfection procedures due to its decontaminating properties. The aim of this study was to determine the antimicrobial efficacy of a plasma decontamination module that included a dielectric barrier discharge for plasma generation. Experimental parameters such as an airflow velocity of 4.5 m/s and microbial contaminations of approximately 6, 000 colony‐forming units (cfu)/m 3 were used to simulate practical conditions of a ventilation system in an operating theater. The apathogenic microorganism Escherichia coli K12 DSM 11250/NCTC 10538 and the multidrug‐resistant strains E. coli 21181 and 21182 (isolated from patients) were tested to determine the antimicrobial efficacy. In summary, the number of cfu was reduced by 31–89% for the tested E. coli strains, whereby E. coli K12 was the most susceptible strain toward inactivation by the designed plasma module. A possible correlation between the number or kind of resistances and susceptibility against plasma was discussed. The inactivation of microorganisms was affected by plasma intensity and size of the plasma treatment area. In addition, the differences of the antimicrobial efficacies caused through the nebulization of microorganisms in front (upstream) or behind (downstream) the plasma source were compared. The presence of ionic wind had no influence on the reduction of the number of cfu for E. coli K12, as the airflow velocity was too high for a successful precipitation, which would be a prerequisite for an increased antimicrobial efficacy. The inactivation of the tested microorganisms confirms the potential of CAP for the improvement of air quality. The scale‐up of this model system may provide a novel tool for an effective air cleaning process. Abstract : The dissemination of pathogenic microorganisms in medical or health care facilities is a life‐threatening problem. In this regard, the developed plasma decontamination module using a cold atmospheric‐pressure plasma as an antimicrobial agent may be a novel tool for an effective air cleaning process. Different parameters such as the intensity of plasma or size and position of the plasma treatment area influenced the antimicrobial efficacy. Furthermore, the influence of the direct contact of plasma and microorganisms was separated from the contact of microorganisms only with plasma‐generated reactive species during indirect treatment. This plasma technology enables the inactivation of microorganisms including multidrug‐resistant strains to improve air quality and to reduce the risk for infections.