Effect of polydiacetylene-based nanosomes on cell viability and endocytosis. (1st April 2019)

• Record Type: Journal Article
• Authors: Park, Kyu Ha; Ku, Minhee; Yoon, Nara; Hwang, Dae Youn; Lee, Jaebeom; Yang, Jaemoon; Seo, Sungbaek
• Is Part Of: Nanotechnology. Volume 30:Number 24(2019)
• Publication Date: 2019-04-01
• Subjects: nanosome -- electrostatic complex -- cell viability -- endocytosis; Nanotechnology -- Periodicals; Nanotechnology -- Periodicals; Nanotechnology; Publications périodiques; Nanotechnologies; Periodicals; 620.5
• DOI: 10.1088/1361-6528/ab0ccf
• Languages: English
• ISSNs: 0957-4484

Abstract

Abstract: Polydiacetylene-based nanoparticles have been developed as nanocarriers for various bio-applications. However, how nanocarriers enter the cell environment and affect cell viability has not yet been considerably explored. In this study, polydiacetylene-based nanoliposomes (nanosomes) were electrostatically complexed with rhodamine fluorophores. Based on real-time cell imaging and cell viability assessment, the most highly polymerized nanosomes were found to be less toxic to cells. Moreover, it was revealed that the rhodamine/polydiacetylene nanosome complex dissociates at cell environment, the polydiacetylene nanosome penetrates into cells, as suggested by the fluorescence observed in confocal microscopy images.