Role of Trpv1 and Trpv4 in surgical incision-induced tissue swelling and Fos-like immunoreactivity in the central nervous system of mice. (21st June 2018)

• Record Type: Journal Article
• Authors: Motojima, Yasuhito; Nishimura, Haruki; Ueno, Hiromichi; Sonoda, Satomi; Nishimura, Kazuaki; Tanaka, Kentaro; Saito, Reiko; Yoshimura, Mitsuhiro; Maruyama, Takashi; Matsuura, Takanori; Suzuki, Hitoshi; Kawasaki, Makoto; Ohnishi, Hideo; Sakai, Akinori; Ueta, Yoichi
• Is Part Of: Neuroscience letters. Volume 678(2018)
• Page Start: 76
• Page End: 82
• Publication Date: 2018-06-21
• Subjects: CeA central amygdala -- HPA hypothalamic pituitary-adrenal -- i.p. intraperitoneal -- Fos-LI Fos-like immunoreactivity -- PB phosphate buffer -- PBST phosphate-buffered saline containing 0.3% Triton X-100 -- PFA paraformaldehyde -- PVN paraventricular nucleus of the hypothalamus -- PVT paraventricular nucleus of the thalamus -- s.c. subcutaneously -- TRP transient receptor potential -- TRPV transient receptor potential vanilloid -- WT wild-type -- 4α-PDD 4α-phorbol 12, 13-didecanoate; Central nervous system -- Incision -- Paraventricular nucleus -- Spinal cord -- Trpv1 -- Trpv4; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2018.05.001
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: TRPV1 and TRPV4 function was completely lost in Trpv1 −/− and Trpv4 −/− mice. Surgical incision-induced paw swelling in mice is not affected by TRPV1 and V4 loss. Surgical incision activates spinal cord and paraventricular nucleus of hypothalamus. Incision-induced Fos in laminae III–IV were increased in Trpv1 −/− and Trpv4 −/− mice. Incision activates laminae III–IV cells specifically in Trpv1 −/− and Trpv4 −/− mice. Abstract: Pain management remains a major concern regarding the treatment of postoperative patients. Transient receptor potential (TRP) channels are considered to be new therapeutic targets for pain control. We investigated whether the genes Trpv1 and Trpv4 are involved in hind paw swelling caused after surgical incision in mice or in incision-induced Fos-like immunoreactivity (Fos-LI) levels in the central nervous system. Mice were divided into four groups: wild-type (WT) control, WT incision, Trpv1 knockout ( Trpv1 −/− ) incision, and Trpv4 knockout ( Trpv4 −/− ) incision. Mice were anesthetized, and only those in the incision, and not control, groups received a surgical incision to their right plantar hind paw. Changes in paw diameter and in Fos-LI levels in the dorsal horn of the spinal cord, paraventricular nucleus of the hypothalamus (PVN), paraventricular nucleus of the thalamus, and central amygdala were evaluated 2 h after the incision. There was no significant difference in the paw diameter among groups. In contrast, in laminae I–II of the dorsal horn of the spinal cord and PVN, Fos-LI was significantly higher in all incision groups than in the WT control group. A significant increase in Fos-positive cells was also observed in the dorsal horn laminae III–IV in Trpv1 −/− and Trpv4 −/− incision groups compared with the WT incision group. Our results indicate that surgical incision activates the PVN and that Trpv1 and Trpv4 might be involved in neuronal activity in the dorsal horn laminae III–IV after surgical incision.