The plastidial metabolite 2‐C‐methyl‐D‐erythritol‐2, 4‐cyclodiphosphate modulates defence responses against aphids. (8th March 2019)

• Record Type: Journal Article
• Authors: Onkokesung, Nawaporn; Reichelt, Michael; Wright, Louwrance P.; Phillips, Michael A.; Gershenzon, Jonathan; Dicke, Marcel
• Is Part Of: Plant, cell and environment. Volume 42:Number 7(2019)
• Page Start: 2309
• Page End: 2323
• Publication Date: 2019-03-08
• Subjects: aphid resistance -- Arabidopsis -- indole glucosinolates -- phloem‐sucking herbivores -- phytohormone signalling -- retrograde signalling -- secondary metabolites; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13538
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Feeding by insect herbivores such as caterpillars and aphids induces plant resistance mechanisms that are mediated by the phytohormones jasmonic acid (JA) and salicylic acid (SA). These phytohormonal pathways often crosstalk. Besides phytohormones, methyl‐ D ‐erythriol‐2, 4‐cyclodiphosphate (MEcPP), the penultimate metabolite in the methyl‐ D ‐erythritol‐4‐phosphate pathway, has been speculated to regulate transcription of nuclear genes in response to biotic stressors such as aphids. Here, we show that MEcPP uniquely enhances the SA pathway without attenuating the JA pathway. Arabidopsis mutant plants that accumulate high levels of MEcPP ( hds3 ) are highly resistant to the cabbage aphid ( Brevicoryne brassicae ), whereas resistance to the large cabbage white caterpillar ( Pieris brassicae ) remains unaltered. Thus, MEcPP is a distinct signalling molecule that acts beyond phytohormonal crosstalk to induce resistance against the cabbage aphid in Arabidopsis. We dissect the molecular mechanisms of MEcPP mediating plant resistance against the aphid B . brassicae . This shows that MEcPP induces the expression of genes encoding enzymes involved in the biosynthesis of several primary and secondary metabolic pathways contributing to enhanced resistance against this aphid species. A unique ability to regulate multifaceted molecular mechanisms makes MEcPP an attractive target for metabolic engineering in Brassica crop plants to increase resistance to cabbage aphids. Abstract : The plastidial metabolite methyl‐ D ‐erythriol‐2, 4‐cyclodiphosphate is a positive regulator of genes in SA signalling and biosynthetic genes in various metabolic pathways that confer resistance to aphids without compromising resistance to caterpillars.