CD14/16 monocyte profiling in juvenile myelomonocytic leukemia. Issue 9 (10th July 2020)

• Record Type: Journal Article
• Authors: Gadgeel, Manisha; Bagla, Shruti; Buck, Steven; Shamoun, Mark; Ravindranath, Yaddanapudi
• Is Part Of: Pediatric blood & cancer. Volume 67:Issue 9(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-07-10
• Subjects: flow cytometry -- juvenile myelomonocytic leukemia -- monocyte subsets; Tumors in children -- Periodicals; Blood -- Diseases -- Periodicals; Cancer in children -- Periodicals; 618.92
• DOI: 10.1002/pbc.28555
• Languages: English
• ISSNs: 1545-5009

Abstract

Abstract: Monocyte subset analysis by flow cytometry has been shown to be a useful diagnostic tool in chronic myelomonocytic leukemia in adults. An increase in the classical monocyte fraction (CD14++/CD16−) greater than 94.0% of total monocytes is considered highly sensitive and specific in distinguishing chronic myelomonocytic leukemia from other myeloproliferative disorders. In a pilot study of juvenile myelomonocytic leukemia cases, we noted that CD14++/CD16− monocyte fraction was >95% in de novo juvenile myelomonocytic leukemia (JMML) with somatic PTPN11 mutations but normal in those with monosomy 7 or Noonan syndrome. Monocyte subgroup profiling by itself is not diagnostic of JMML but may distinguish molecular subgroups within JMML.