Comparison of the levels of hepatocyte growth factor and vascular endothelial growth factor in aqueous fluid and serum with grades of retinopathy in patients with diabetes mellitus. Issue 7 (1st July 1999)

• Record Type: Journal Article
• Authors: Shinoda, Kei; Ishida, Susumu; Kawashima, Shinichi; Wakabayashi, Toshiko; Matsuzaki, Tadayuki; Takayama, Michiyo; Shinmura, Ken; Yamada, Masakazu
• Is Part Of: British journal of ophthalmology. Volume 83:Issue 7(1999)
• Page Start: 834
• Page End: 837
• Publication Date: 1999-07-01
• Subjects: aqueous humour -- diabetic retinopathy -- hepatocyte growth factor -- neovascularisation -- vascular endothelial growth factor; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.83.7.834
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : AIMS: To determine the relation between the stages of diabetic retinopathy (DR) and the levels of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in aqueous fluid and serum. METHODS: Levels of HGF and VEGF in serum and aqueous humour obtained during ocular surgery were measured by enzyme linked immunosorbent assay in 58 diabetic patients with 32 non-diabetic patients (NDM) as controls. The patients with diabetes were classified into three groups according to the stage of DR: no DR (NDR; 15 cases), non-proliferative DR (NPDR; six cases), and proliferative DR (PDR; 37 cases). RESULTS: No significant differences were found between any of the groups in serum concentrations of HGF or VEGF. The aqueous HGF levels increased with the stage of DR: NDM, median 397 pg/ml, range 133–930 pg/ml; NDR, 371 pg/ml, 142–1536 pg/ml; NPDR, 455 pg/ml, 162–1007 pg/ml; and PDR, 638 pg/ml, 187–2222 pg/ml. The aqueous VEGF levels in PDR (median 212 pg/ml, range 14–1216 pg/ml) were significantly higher than in NDM (105 pg/ml, 9–203 pg/ml), but aqueous HGF concentrations were unrelated to those of VEGF. CONCLUSION: The results of the present study suggest that both HGF and VEGF present in the ocular tissues may play important roles in the progression of DR.