1213 Plasmatic micrornas profile expression in malignant pleural mesothelioma. (24th April 2018)

• Record Type: Journal Article
• Authors: Aguilar, G; Jiménez, C; Juárez, C; Gómez, R; Rosas, H; Cruz, D
• Is Part Of: Occupational and environmental medicine. Volume 75(2018)Supplement 2
• Page Start: A406
• Page End: A407
• Publication Date: 2018-04-24
• Subjects: Asbestos -- microRNAs -- RT-qPCR; Medicine, Industrial -- Periodicals; Environmental health -- Periodicals; 616.980305
• DOI: 10.1136/oemed-2018-ICOHabstracts.1161
• Languages: English
• ISSNs: 1351-0711

Abstract

Abstract : Introduction: Malignant Pleural Mesothelioma (MPM) is an aggressive cancer caused by occupational, environmental and indirect exposure to asbestos, material that some countries have already prohibited. Survival is less than 1 year. Diagnosis is currently a challenge and the search for early, single or combined diagnostic biomarkers continues to be performed on non-invasive samples. MicroRNAs are circulating released molecules whose expression is altered in some types of cancer, making them good diagnostic candidates in the MMP. Methods: A descriptive study was carried out in plasma of 6 cases of MPM and 6 controls without exposure to asbestos matched by age and sex. The samples come from a previous case-control study conducted in Mexico from 2011 to 2016. We evaluated 384 plasma microRNAs by means of RT-qPCR using the platform and QIAgen. The analysis of results was performed with the GeneGlobe program. Results hsa-miR-1587, hsa-miR-19b-1–5 p, hsa-miR-93–3 p, hsa-miR-21–3 p, hsa-let-7i-3p, hsa-miR-194–5 p, hsa-miR-1280, hsa-miR-18a-3p, hsa-miR-133a-3p, hsa-miR-4286, hsa-miR-2467–3 p, had altered expression in cases and controls Discussion: While some microRNAs found in this study have been associated with kidney disease, hypertension, endometriosis, liver cancer, diabetes, colorectal cancer, gastric and esophageal cancer; The microRNA hsa-let-7i-3p associated with lung cancer are specially interest, miR-194–5 p deregulated in tumorogenesis and miR-1280 modulates cell growth, so the next step will be to validate them in a new collection of samples and perform the network of interaction in the metabolic pathways.