The effect of ambient temperature on the anti-D assay using the Auto Analyzer. Issue 5 (May 1974)

• Record Type: Journal Article
• Authors: Gunson, H. H.; Phillips, P. K.; Stratton, F.
• Is Part Of: Journal of clinical pathology. Volume 27:Issue 5(1974)
• Page Start: 356
• Page End: 358
• Publication Date: 1974-05
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.27.5.356
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Using the AutoAnalyzer, the percentage agglutination effected by the anti-D antisera studied showed a varied dependence on the ambient temperature over the manifold subsequent to the incubation period at 37°C. This leads to assays which are a function of the ambient temperature. It is suggested that the entry of a relatively large volume of rouleaux-dispersing agent results in an elution of bound antibody to a new position of equilibrium, the shift being dependent on the particular equilibrium constant of the antibody and the rate of its attainment on the ambient temperature. A constant ambient temperature will lead to greater accuracy of anti-D assay.