Lactic acid bacteria inhibits quorum sensing and biofilm formation of Pseudomonas aeruginosa strain JUPG01 isolated from rancid butter. (September 2021)

• Record Type: Journal Article
• Authors: Aman, Mohammed; Aneeqha, N.; Bristi, K.; Deeksha, J.; Afza, Noor; Sindhuja, V.; Shastry, Rajesh P.
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 36(2021)
• Publication Date: 2021-09
• Subjects: Quorum quenching -- Lactic acid bacteria -- Secondary metabolites -- Exopolysaccharides -- Anti-biofilm; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2021.102115
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: The majority of pathogenic bacteria utilize cell to cell communication mechanisms to regulate the cell density and expression of virulence factors. Therefore, quorum sensing system could be one of the targets for antimicrobial strategy to control quorum sensing regulated phenotypes in the pathogenic bacteria. Several lactic acid bacteria produce peptides and secondary metabolites having antimicrobial properties. In the present study, exopolysaccharides (EPS) and secondary metabolites from the Lactic Acid Bacteria (LABs) isolated from various sources such as cow milk, sourdough, chicken intestine, buttermilk, roasted meat and idly batter were screened for quorum quenching properties against P. aeruginosa strain JUPG01isolated from rancid butter. The results revealed that EPS from LABs were not significant against screened quorum quenching activity. However, the secondary metabolites extracted from Pediococcus pentosaceus strain BS-2 and Lactobacillus fermentum strain BM-2isolated from cow milk exhibited significant quorum quenching activity. Furthermore, the strains BS-2 and BM-2 revealed significant anti-biofilm activity against P. aeruginosa strain JUPG01. This study highlights the biotechnological applications of LABs in the eradication of Pseudomonas species associated with dairy food contamination and spoilage. Graphical abstract: Image 1 Highlights: Isolation of Lactic acid bacteria (LABs) from different sources. Isolation of Pseudomonas aeruginosa strain JUPG01 from rancid butter. Screening of quorum quenching potentiality of LABs against P. aeruginosa strain JUPG01. Molecular identification of bioactive strains of Lactic acid bacteria. Extraction, elucidation and partial characterization of bioactive metabolites using FTIR analysis.