Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types. Issue 6 (25th May 2005)

• Record Type: Journal Article
• Authors: Malta-Vacas, J; Aires, C; Costa, P; Conde, A R; Ramos, S; Martins, A P; Monteiro, C; Brito, M
• Is Part Of: Journal of clinical pathology. Volume 58:Issue 6(2005)
• Page Start: 621
• Page End: 625
• Publication Date: 2005-05-25
• Subjects: eIF, eukaryotic initiating factor -- eRF, eukaryotic release factor -- PCR, polymerase chain reaction -- RT, reverse transcription; gastric cancer -- eRF3/GSPT1 -- gene expression -- real time polymerase chain reaction -- translation machinery; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.2004.021774
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Background: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p < 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription.