3D Bioprinting of Oxygenated Cell‐Laden Gelatin Methacryloyl Constructs. Issue 15 (16th June 2020)

• Record Type: Journal Article
• Authors: Erdem, Ahmet; Darabi, Mohammad Ali; Nasiri, Rohollah; Sangabathuni, Sivakoti; Ertas, Yavuz Nuri; Alem, Halima; Hosseini, Vahid; Shamloo, Amir; Nasr, Ali S.; Ahadian, Samad; Dokmeci, Mehmet R.; Khademhosseini, Ali; Ashammakhi, Nureddin
• Other Names: Zhang Yu Shrike guestEditor.
• Is Part Of: Advanced healthcare materials. Volume 9:Issue 15(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-16
• Subjects: 3D bioprinting -- bioinks -- calcium peroxide -- cardiomyocytes -- hypoxia -- oxygen; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201901794
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O2 ) to transplanted cells via an O2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties lead to the production of a robust bioink suitable for 3D bioprinting and controlled O2 release. In addition, O2 release, bioprinting conditions, and mechanical performance of hydrogels having different CPO concentrations are characterized. As a proof of concept study, fibroblasts and cardiomyocytes are bioprinted using CPO containing GelMA bioink. Viability and metabolic activity of printed cells are checked after 7 days of culture under hypoxic condition. The results show that the addition of CPO improves the metabolic activity and viability of cells in bioprinted constructs under hypoxic condition. Abstract : This study presents a novel approach to develop oxygenated bioinks, using oxygen generating calcium peroxide particles. Cardiomyocyte‐laden oxygenated and 3D printed tissue constructs developed in this study can significantly support cell survival when applied in hypoxic conditions. They can also be delivered using minimally invasive approach to ischemic hearts.