A Gas Chromatography‐Molecular Rotational Resonance Spectroscopy Based System of Singular Specificity. (19th November 2019)

• Record Type: Journal Article
• Authors: Armstrong, Daniel W.; Talebi, Mohsen; Thakur, Nimisha; Wahab, M. Farooq; Mikhonin, Alexander V.; Muckle, Matt T.; Neill, Justin L.
• Is Part Of: Angewandte Chemie. Volume 132:Number 1(2020)
• Page Start: 198
• Page End: 202
• Publication Date: 2019-11-19
• Subjects: Biologischer Abbau -- Isotopologen/Isotopomere -- Massenspektrometrie -- Rotationsspektroskopie -- Verbindungsspezifische Isotopenanalyse; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201910507
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: We designed and demonstrated the unique abilities of the first gas chromatography–molecular rotational resonance spectrometer (GC‐MRR). While broadly and routinely applicable, its capabilities can exceed those of high‐resolution MS and NMR spectroscopy in terms of selectivity, resolution, and compound identification. A series of 24 isotopologues and isotopomers of five organic compounds are separated, identified, and quantified in a single run. Natural isotopic abundances of mixtures of compounds containing chlorine, bromine, and sulfur heteroatoms are easily determined. MRR detection provides the added high specificity for these selective gas‐phase separations. GC‐MRR is shown to be ideal for compound‐specific isotope analysis (CSIA). Different bacterial cultures and groundwater were shown to have contrasting isotopic selectivities for common organic compounds. The ease of such GC‐MRR measurements may initiate a new era in biosynthetic/degradation and geochemical isotopic compound studies. Abstract : Superspezifische Trennungen : Eine beispiellose Strukturspezifität für isobare, isotopische und isomere Verbindungen wird durch die erste Kombination von hocheffizienten Trennungen mit hochauflösender Rotationsresonanzspektroskopie erreicht. Selbst eng verwandte Spezies in kleinsten Mengen komplexer Mischungen können schnell und eindeutig identifiziert und quantifiziert werden.