Simple method for the preparation of single cell suspensions from normal and tumorous rat colonic mucosa. Issue 5 (May 1977)

• Record Type: Journal Article
• Authors: Perret, V; Lev, R; Pigman, W
• Is Part Of: Gut. Volume 18:Issue 5(1977)
• Page Start: 382
• Page End: 385
• Publication Date: 1977-05
• Subjects: Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.18.5.382
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : Viable single cell suspensions from rat colonic epithelium were obtained by using phosphate buffered saline containing 0-2 M mannitol. The method, which requires no prior enzyme treatment, provides undamaged cells in high yield within one hour. The procedure was also applied to neoplastic rat colonic tissue, which was induced by repeated intrarectal infusion of N-methyl-N-nitrosourea. Comparison between normal and neoplastic cells has shown that the latter have a higher nucleus: cytoplasm ratio and a higher metabolic activity.