Slilica, Hyaluronate, and Alveolar Macrophage Functional Differentiation. (1st March 2003)

• Record Type: Journal Article
• Authors: Bodo, Maria; Bellocchio, Silvia; Bellucci, Catia; Balloni, Stefania; Pietrella, Donatella; Sbaraglia, Giovanni; Muzi, Giacomo; Lilli, Cinzia; Lumare, Alessandro; Dell'Omo, Marco; Abbritti, Giuseppe
• Is Part Of: Journal of investigative medicine. Volume 51:Number 2(2003)
• Page Start: 95
• Page End: 103
• Publication Date: 2003-03-01
• Subjects: NR8383 cell lines -- silica particles -- extracellular matrix -- nitric oxide -- Candida -- phagocytosis -- fibrogenic cytokines; Clinical medicine -- Periodicals; Medicine -- Research -- Periodicals; Medicine; Research -- United States; Clinical medicine; Medicine -- Research; Periodicals; 616.075
• DOI: 10.1136/jim-51-02-11
• Languages: English
• ISSNs: 1081-5589

Abstract

Abstract : Background: Silicosis is mediated by macrophages, their soluble mediators, and extracellular matrix molecules. In this study, we investigated the effects of silica and/or hyaluronate (HA) on several alveolar macrophage responses. Methods: We evaluated glycosaminoglycan (GAG) production by radiolabeled precursors, nitric oxide (NO) release by its oxidation product, phagocytic activity by Candida albicans internalization, and the secretion of two fibrogenic cytokines, tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β, by specific assays. Results: Silica significantly reduced GAG secretion, particularly HA secretion. Alone, it decreased Candida uptake; associated with HA, it enhanced the reduction. Silica and Candida reduced NO release, which was not significantly affected when silica- or Candida -exposed cells were also treated with HA. TNF-α and TGF-β activities were stimulated by silica but reduced by HA. Conclusions: The results suggest that silica and HA modify alveolar macrophage functional differentiation. Silica- and HA-induced modifications of the microenvironment could determine whether the response proceeds toward healing and repair or toward lung chronic pathology.