Challenging diagnosis of gastrointestinal tuberculosis made simpler with multi-targeted loop-mediated isothermal amplification assay. Issue 8 (August 2020)

• Record Type: Journal Article
• Authors: Sharma, Megha; Sinha, Saroj Kant; Sharma, Megha; Singh, Anupam Kumar; Samanta, Jayanta; Sharma, Aman; Kochhar, Rakesh; Sharma, Kusum
• Is Part Of: European journal of gastroenterology & hepatology. Volume 32:Issue 8(2020)
• Publication Date: 2020-08
• Subjects: IS6110 -- MPB64 -- nucleic acid amplification -- turn-around time; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; Digestive organs -- Diseases; Liver -- Diseases; Periodicals; 616.33
• DOI: 10.1097/MEG.0000000000001765
• Languages: English
• ISSNs: 0954-691X

Abstract

Abstract : Background: Prompt and accurate diagnosis of gastrointestinal tuberculosis (GITB) is highly challenging. Current conventional techniques lack sensitivity and are time-consuming. Multi targeted loop-mediated isothermal amplification (LAMP) using two targets ( IS 6110 and MPB64) is a promising technique for rapid diagnosis of TB. Methods: LAMP test using IS 6110 and MPB64 targets for M. tuberculosis complex was performed on ileocecal biopsy samples of 35 clinically suspected patients of GITB and 30 ileocecal biopsy samples of non-tuberculosis control subjects. Results of IS 6110 LAMP and MPB64 LAMP were compared with IS 6110 PCR and culture Inference: Overall LAMP test (using any of the two targets) had a sensitivity and specificity of 100 and 100%, respectively, for confirmed GITB (five culture positive) cases and 85.71 and 100%, respectively, for clinically suspected 30 ileocecal biopsy samples of GITB. Sensitivity of IS 6110 LAMP, MPB64 LAMP and IS 6110 PCR for clinically suspected cases was 22 (73.33%), 24 (80%) and 21 (70%), respectively. In total 35 GITB patients, the overall sensitivity of microscopy, culture, IS 6110 PCR, IS 6110 LAMP, MPB64 LAMP and the multi-targeted LAMP assay (if any of the two targets were used) were 5.71, 14.28, 68.57, 74.28, 82.85 and 85.71%, respectively. Specificity of all the tests was 100%. There were three cases which were missed by IS 6110 LAMP and two cases by MPB64 LAMP. Conclusion: Multi-targeted LAMP is a promising technique for rapid and accurate diagnosis of GITB.