The Mafb cleft‐associated variant H131Q is not required for palatogenesis in the mouse. Issue 10 (27th March 2021)

• Record Type: Journal Article
• Authors: Paul, Brian J.; Palmer, Kristina J.; Rhea, Lindsey; Carlson, Melissa; Sharp, Jocelyn C.; Pratt, C. Herbert; Murray, Stephen A.; Dunnwald, Martine
• Is Part Of: Developmental dynamics. Volume 250:Issue 10(2021)
• Page Start: 1463
• Page End: 1476
• Publication Date: 2021-03-27
• Subjects: craniofacial -- development -- Mafb -- mouse -- mutation; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.327
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract: Background: Orofacial clefts (OFCs) are common birth defects with complex etiology. Genome wide association studies for OFC have identified SNPs in and near MAFB . MAFB is a transcription factor critical for structural development of digits, kidneys, skin, and brain. MAFB is also expressed in the craniofacial region. Previous sequencing of MAFB in a Filipino population revealed a novel missense variant significantly associated with an increased risk for OFC. This MAFB variant, leading to the amino acid change H131Q, was knocked into the mouse Mafb, resulting in the Mafb H131Q allele. The Mafb H131Q construct was engineered to allow for deletion of Mafb (" Mafb del "). Results: Mafb del/del animals died shortly after birth. Conversely, Mafb H131Q/H131Q mice survived into adulthood at Mendelian ratios. Mafb del/del and Mafb H131Q/H131Q heads exhibited normal macroscopic and histological appearance at all embryonic time points evaluated. The periderm was intact based on expression of keratin 6, p63, and E‐cadherin. Despite no effect on craniofacial morphogenesis, H131Q inhibited the Mafb‐dependent promoter activation of Arhgap29 in palatal mesenchymal, but not ectodermal‐derived epithelial cells in a luciferase assay. Conclusions: Mafb is dispensable for murine palatogenesis in vivo, and the cleft‐associated variant H131Q, despite its lack of morphogenic effect, altered the expression of Arhgap29 in a cell‐dependent context. Key Findings: Mafb is dispensable for palatogenesis in the mouse Cleft associated variant H131Q alters the expression of Arhgap29 in a cell‐dependent context