A novel 2A‐peptide‐containing plasmid to generate stable Perkinsusmarinus cells expressing organelle‐targeted genes. (4th July 2021)

• Record Type: Journal Article
• Authors: Sakamoto, Hirokazu; Kita, Kiyoshi; Matsuzaki, Motomichi
• Is Part Of: Journal of eukaryotic microbiology. Volume 68:Number 5(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-04
• Subjects: alternative oxidase -- dinoflagellates -- drug selection -- genetic manipulation -- membrane‐bound organelle -- polycistronic plasmid; Protista -- Periodicals; Eukaryotic cells -- Periodicals; Microbiology -- Periodicals; 579.05
• DOI: 10.1111/jeu.12861
• Languages: English
• ISSNs: 1066-5234

Abstract

Abstract: Genetic manipulation techniques for marine protists are not well‐established, despite immense efforts. However, Perkinsus marinus is an exception and can be developed as a genetically tractable model organism for related protists. Here, we designed a new plasmid for P . marinus that allows two proteins from a single mRNA to be differently localized using a self‐cleaving 2A peptide. This enabled us to establish a stable transfectant expressing a mitochondrially targeted fluorescent protein. The system can be applied to any protein in theory and would make a powerful tool for investigating unique organelles in P . marinus and related dinoflagellates.