Antidiabetic activity of Musa x paradisiaca extracts in streptozotocin-induced diabetic rats and chemical characterization by HPLC-DAD-MS. (23rd May 2020)

• Record Type: Journal Article
• Authors: Vilhena, R.O.; Figueiredo, I.D.; Baviera, A.M.; Silva, D.B.; Marson, B.M.; Oliveira, J.A.; Peccinini, R.G.; Borges, I.K.; Pontarolo, R.
• Is Part Of: Journal of ethnopharmacology. Volume 254(2020)
• Publication Date: 2020-05-23
• Subjects: Banana -- Hydroxycinnamic acid derivatives -- HPLC-DAD-MS -- Anthocyanins -- Diabetes mellitus; HPLC-DAD-MS high-performance liquid chromatography coupled to diode-array detection and mass spectrometry -- AFE aqueous flower extract -- MFF methanolic flower fraction -- ABE aqueous bract extract -- MBF methanolic bract fraction -- AqE aqueous extract -- MeF methanolic -- N normal group -- D untreated diabetic group -- DAFE diabetic group treated with aqueous flower extract -- DMFF diabetic group treated with methanolic flower fraction -- DABE diabetic group treated with aqueous bract extract -- DMBF diabetic group treated with methanolic bract fraction -- Dp-CA diabetic group treated with p-coumaric acid -- DINS diabetic group treated with insulin -- b.w. body weight; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2020.112666
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: The Musa x paradisiaca L. inflorescence, known as banana blossom or banana heart, is used in traditional medicine for the treatment of diabetes mellitus. Aim of the study: The aim of the study was to investigate the antidiabetic activity of aqueous extracts and fractions prepared from the bracts and flowers of Musa x paradisiaca in streptozotocin (STZ)-induced diabetic rats and to chemically characterize the extracts. Materials and methods: Standard aqueous extracts of the flowers, bracts, and their fractions were prepared and their chemical composition was determined tentatively by high-performance liquid chromatography coupled to diode-array detection and mass spectrometry (HPLC-DAD-MS). Changes in fasting glycemia and oral glucose tolerance were evaluated in STZ-induced diabetic rats (n = 8) treated with aqueous extracts of Musa x paradisiaca (200 mg/kg) for 20 days. Results: Chemical analyses detected 21 compounds and 17 metabolites were identified, among which were glycosylated and acetylated phenylpropanoids of p -coumaric acid and caffeic acid, as well as a glycosylated flavonol and anthocyanins. Following 15 days of treatment, the bract aqueous extracts and the methanolic fraction of the flower had significant effects on the glycemic profile after glucose load in diabetic rats as compared with the untreated diabetic group. Conclusions: The results of the present study show the antidiabetic potential of extracts of the flowers and bracts of M. x paradisiaca . Graphical abstract: Image 1