Coexpression gene network analysis of cold‐tolerant Solanum commersonii reveals new insights in response to low temperatures. Issue 5 (2nd July 2021)

• Record Type: Journal Article
• Authors: Esposito, Salvatore; Aversano, Riccardo; Bradeen, James; D'Amelia, Vincenzo; Villano, Clizia; Carputo, Domenico
• Is Part Of: Crop science. Volume 61:Issue 5(2021)
• Page Start: 3538
• Page End: 3550
• Publication Date: 2021-07-02
• Subjects: Crop science -- Periodicals; Cultures -- Périodiques; Cultures de plein champ -- Périodiques; Crop science; Nutzpflanzen; Zeitschrift; Pflanzenbau; Periodicals; 633
• DOI: 10.1002/csc2.20473
• Languages: English
• ISSNs: 0011-183X

Abstract

Abstract: Among abiotic stressors, cold is one of the most harmful for the cultivated potato ( Solanum tuberosum L.), a frost‐sensitive crop. RNA sequencing (RNA‐seq) profiling of two different clones of wild potato ( S. commersonii Dun.) contrasting in their capacity to withstand low temperatures revealed a higher number of differentially expressed genes (DEGs) under nonacclimated conditions (NAC) in tolerant clone cmm1T vs. the susceptible cmm6‐6 (1, 002 and 8, 055 DEGs, respectively). By contrast, the number of DEGs was much more comparable when both genotypes were under acclimated conditions (AC). Indeed, a total of 5, 650 and 8, 936 DEGs were detected in the tolerant genotype vs. the susceptible. Gene ontology (GO) classification under NAC showed a significant role for transcription regulation, lignin catabolic genes, and regulation of plant type secondary cell wall in the cold‐tolerant genotypes, suggesting an important role in conferring tolerance response. By contrast, response to stress and response to stimuli were enriched GO categories in both clones under AC. Unsigned weighted correlation networks analysis (WGCNA) allowed identification of coexpressed hub genes with possible main regulatory functions and major impacts on the phenotype. Among those identified, we clarified the role of CBF4 . This gene showed contrasting expression profiles in the two clones under NAC, being induced in cold‐tolerant cmm1T but suppressed in susceptible cmm6‐6. By contrast, under AC, CBF4 was upregulated in both clones. Our study provides a global understanding of mechanisms involved following exposure to NAC and AC in S. commersonii . The mechanisms described here will inform future investigations for detailed validation in studies regarding cold tolerance in plants. Core Ideas: Differences in expression patterns of two Solanum clones contrasting in their cold response. WGCNA identifies candidate genes associated with cold tolerance under NAC and AC. We documented the role for a CBF ‐dependent pathway under both AC and NAC.