The miR-122 inhibition alleviates lipid accumulation and inflammation in NAFLD cell model. (3rd September 2021)

• Record Type: Journal Article
• Authors: Hu, Yiyi; Du, Guoping; Li, Guohua; Peng, Xuetao; Zhang, Zhiqiao; Zhai, Yingji
• Is Part Of: Archives of physiology and biochemistry. Volume 127:Number 5(2021)
• Page Start: 385
• Page End: 389
• Publication Date: 2021-09-03
• Subjects: Nonalcoholic fatty liver disease -- lipid accumulation -- inflammation -- miR-122 -- TLR4/MyD88/NF-κBp65; Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.1080/13813455.2019.1640744
• Languages: English
• ISSNs: 1381-3455

Abstract

Abstract: Background: Accumulating evidence showed that the expression of miR-122 was abnormal in NAFLD patients; however, the role of miR-122 on lipid accumulation and inflammation in NAFLD is not clear. Methods: RT-qPCR was applied to detect the expression levels of miR-122 and pro-inflammatory cytokines following transfected with miR-122 inhibitor or treated with oleic acid (OA). Detection of lipid accumulation was performed by triglyceride content test and oil red o staining assay. Western blotting was applied to detect the protein levels of TLR7, TLR4, MyD88 and NF-κBp65. Results: We found that the OA promoted lipid accumulation and pro-inflammatory cytokines secretion and activated TLR4/MyD88/NF-κBp65 signalling pathway, which were restored following transfected with miR-122 inhibitor. Conclusions: These results suggested that miR-122 inhibition alleviates lipid accumulation and inflammation in L02 cell induced by OA may through inhibiting TLR4/MyD88/NF-κBp65 signalling pathway. The protective mechanism of miR-122 inhibition in NAFLD must be explored in future studies.