Expression of the IL-2R in Human Podocytes and the Effect of Activation on Autophagy and Apoptosis. (6th October 2021)

• Record Type: Journal Article
• Authors: Stewart, Tyrus; Zea, Arnold; Aviles, Diego
• Is Part Of: Fetal and pediatric pathology. Volume 40:Number 5(2021)
• Page Start: 369
• Page End: 377
• Publication Date: 2021-10-06
• Subjects: Podocytes -- IL-2 receptor -- apoptosis -- autophagy; Pathology, Molecular -- Periodicals; Pediatrics -- Periodicals; Molecular biology -- Periodicals; Pediatric pathology -- Periodicals; Fetal Diseases -- pathology -- Periodicals; Infant, Newborn, Diseases -- pathology -- Periodicals; Pediatrics -- Periodicals; 618.92007
• DOI: 10.1080/15513815.2019.1710793
• Languages: English
• ISSNs: 1551-3815

Abstract

Abstract: Background: Interleukin 2 (IL-2) treatment is associated with proteinuria. Materials and Methods: A conditionally immortalized human podocyte cell line was used to investigate expression of the podocyte specific marker podocin, IL-2R alpha (IL-2Rα), apoptosis marker Bax, and autophagy markers LC3I AND LC3II, determined by quantitative immunoblotting, following 24, 48, and 72 hours of IL-2 stimulation, comparing them to unstimulated cells. Results: Podocin was expressed at all time points. IL-2Rα expression was increased after 24 and 72 hrs ( p = 0.0014, p = 0.0139) and decreased after 48 hours ( p = 0.0445). Bax, LC3I, and LC3II were increased after 24 hrs ( p = 0.0094, p = 0.0016, p = 0.0004) and 48 hrs ( p = 0.0072, p = 0.0024, p = 0.0087). Conclusion: Human podocytes express the IL-2R and activation results in increased autophagy and apoptosis.