Liquid Tissue™: proteomic profiling of formalin-fixed tissues. (June 2005)

• Record Type: Journal Article
• Authors: Prieto, Darue A.; Hood, Brian L.; Darfler, Marlene M.; Guiel, Thomas G.; Lucas, David A.; Conrads, Thomas P.; Veenstra, Timothy D.; Krizman, David B.
• Is Part Of: Biotechniques. Volume 38:Number 6(2005)Supplement
• Page Start: S32
• Page End: S35
• Publication Date: 2005-06
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/05386SU06
• Languages: English
• ISSNs: 0736-6205

Abstract

Identification and quantitation of candidate biomarker proteins in large numbers of individual tissues is required to validate specific proteins, or panels of proteins, for clinical use as diagnostic, prognostic, toxicological, or therapeutic markers. Mass spectrometry (MS) provides an exciting analytical methodology for this purpose. Liquid Tissue™ MS protein preparation allows researchers to utilize the vast, already existing, collections of formalin-fixed paraffin-embedded (FFPE) tissues for the procurement of peptides and the analysis across a variety of MS platforms.