CydA, spdC, and mroQ are novel genes involved in the plasma coagulation of Staphylococcus aureus. (21st July 2021)

• Record Type: Journal Article
• Authors: Luo, Dong; Wang, Wei; Chen, Qiang; Peng, Linfeng; Hu, Xiaomei; Chen, Kaisen
• Is Part Of: Microbiology and immunology. Volume 65:Number 9(2021)
• Page Start: 383
• Page End: 391
• Publication Date: 2021-07-21
• Subjects: cydA -- mroQ -- plasma agglutination -- spdC -- Staphylococcus aureus; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12922
• Languages: English
• ISSNs: 0385-5600

Abstract

Abstract: Coagulation is a critical pathogenic process in Staphylococcus aureus . Although the agglutination of S. aureus has been studied for a long time, the genes involved in this process are not completely clear. We performed tube agglutination and dynamic turbidimetry tests to identify novel genes involved in reduced plasma coagulation. A total of 15 genes were identified, including coa, clfA, vwbp, saeS, agrA, trpC, spdC, mroQ, cydA, qoxC, sucC, pyrP, menH, threonine aldolase, and truncated transposase for IS1272 . The functions of these genes include bicomponent regulation, membrane transport, energy metabolism and biosynthesis, respectively. cydA, spdC, and mroQ genes were further studied by gene knockout and complementation. Results of gene knockout and complementation and real‐time‐qPCR proved that cydA, spdC, and mroQ genes are necessary for plasma coagulation. Furthermore, the survival ability of 7 day mice decreased significantly when cydA, spdC, and mroQ genes had been knocked out.