MiRNA expression analysis in the human heart: Undifferentiated progenitors vs. bioptic tissues—Implications for proliferation and ageing. Issue 18 (13th August 2021)

• Record Type: Journal Article
• Authors: Iannolo, Gioacchin; Sciuto, Maria Rita; Cuscino, Nicola; Carcione, Claudia; Coronnello, Claudia; Chinnici, Cinzia Maria; Raffa, Giuseppe Maria; Pilato, Michele; Conaldi, Pier Giulio
• Is Part Of: Journal of cellular and molecular medicine. Volume 25:Issue 18(2021)
• Page Start: 8687
• Page End: 8700
• Publication Date: 2021-08-13
• Subjects: cardiac repair -- cardiovascular -- Heart -- miRNAs -- myocardial infarction -- NGS; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.16824
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: In developed countries, cardiovascular diseases are currently the first cause of death. Cardiospheres (CSs) and cardiosphere‐derived cells (CDCs) have been found to have the ability to regenerate the myocardium after myocardial infarction (MI). In recent years, much effort has been made to gain insight into the human heart repair mechanisms, in which miRNAs have been shown to play an important role. In this regard, to elucidate the involvement of miRNAs, we evaluated the miRNA expression profile across human heart biopsy, CSs and CDCs using microarray and next‐generation sequencing (NGS) technologies. We identified several miRNAs more represented in the progenitors, where some of them can be responsible for the proliferation or the maintenance of an undifferentiated state, while others have been found to be downregulated in the undifferentiated progenitors compared with the biopsies. Moreover, we also found a correlation between downregulated miRNAs in CSs/CDCs and patient age (eg miR‐490) and an inverse correlation among miRNAs upregulated in CSs/CDCs (eg miR‐31).