Double‐Sided Graphene‐Enhanced Raman Scattering and Fluorescence Quenching in Hybrid Biological Structures. Issue 9 (31st July 2021)

• Record Type: Journal Article
• Authors: Sarau, George; Daniel, Christoph; Heilmann, Martin; Leuchs, Gerd; Amann, Kerstin; Christiansen, Silke H.
• Is Part Of: Advanced materials technologies. Volume 6:Issue 9(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-31
• Subjects: amino acids -- fluorescence quenching -- graphene‐enhanced Raman scattering -- kidney tissues -- photobleaching; Materials science -- Periodicals; Technological innovations -- Periodicals; Materials science; Technological innovations; Periodicals; 620.1105
• DOI: 10.1002/admt.202100385
• Languages: English
• ISSNs: 2365-709X

Abstract

Abstract: Due to their large contact and loading surfaces as well as high sensitivities to chemical changes, graphene‐based materials (GBMs) are increasingly being employed into novel nanomedicine technologies. Here biomolecule—monolayer graphene—kidney tissue hybrid structures are studied using mapping micro‐Raman and fluorescence spectroscopies. Because in this configuration graphene interacts with molecules on both sides, a double‐sided graphene‐enhanced Raman scattering (GERS) effect up to ≈ 10.1 is found for biomolecules adsorbed on graphene and amino acids in the kidney tissue below graphene. Moreover, graphene causes an efficient autofluorescence quenching (FLQ) up to ≈ 20% emitted by the kidney tissue. Despite the complexity of such layered materials, the intriguing simultaneous occurrence of double‐sided GERS (a new development of GERS) and FLQ phenomena can be well explained by suitable molecular structure and energy level alignment between molecules and graphene. These result in effective charge transfer mediated by non‐covalent interactions as indicated by correlative strain, doping, and defect analyses in graphene based on the Raman data and energy level calculations. Last, the advantages of using graphene over standard photobleaching are demonstrated. This work can be extended to other macromolecular entities toward integrating GBMs in versatile drug delivery, imaging, and sensing devices. Abstract : Double‐sided graphene‐enhanced Raman scattering and autofluorescence quenching are simultaneously observed in hybrid biomolecular‐graphene structures. These results are explained by low strain and defect density levels in graphene along with its p‐type doping ensuring a proper energy alignment between Fermi level of graphene and molecular orbital levels of top biomolecules and bottom amino acids in the kidney tissue.