AB1238 A comparison between automated and manual methods for detection antinuclear autoantibodies on human epithelial-2 cells. (23rd January 2014)

• Record Type: Journal Article
• Authors: Novikov, A.A.; Alexandrova, E.N.; Roggenbuck, D.; Nasonov, E.L.
• Is Part Of: Annals of the rheumatic diseases. Volume 71(2012)Supplement 3
• Page Start: 708
• Page End: 708
• Publication Date: 2014-01-23
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2012-eular.1234
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: A variety of antinuclear antibodies (ANAs) are found in serum samples obtained from patients with rheumatic autoimmune diseases 1 . Identification of the corresponding antigens and measurement of the antibody titer are useful for diagnosis and to inform about the progress and prognosis of the disease 2-4 . The current gold standard method for ANA detection is manual indirect immunofluorescence (IIF) on human epithelial-2 (HEp-2) cells 5 . Objectives: Automation of ANAs IIF interpretation including pattern recognition can improve intra- and inter- laboratory assay reproducibility. Comparing automated and visual interpretation of ANAs the usefulness for laboratory diagnostics was investigated. Methods: Autoantibody detection by IIF on HEp-2 cells was performed in 234 serums of patients witch suspected and confirmed rheumatic disease. Automated evaluation ("Aklides platform", Medipan GmbH and "ANA HEp-2 Plus" kit, Generic Assays GmbH) were compared with visual interpretations. The screening dilution was 1:160. Results: Automated method of recognition positive/negative results showed a moderate agreement with visual interpretation: kappa=0, 5; case of mismatch is 30 (13%). The automated identification of the pattern (n=83, mixed patterns were excluded from this part of the study - this option is not available when using this automated platform) demonstrated a moderate agreement with visual assessment kappa=0, 6; discrepant results: 22 (26, 5%). Conclusions: Use of this automated platform for ANAs screening provides reliable identification of negative/positive samples and main nuclear patterns. References: Nakabayashi T, Kumagai T, PhD, Yamauchi K et al. Evaluation of the Automatic Fluorescent Image Analyzer, Image Titer, for Quantitative Analysis of Antinuclear Antibodies. Am J Clin Pathol 2001;115:424-429. Notman DD, Kurata N, Tan EM. Profiles of antinuclear antibodies in systemic rheumatic diseases. Ann Intern Med.1975; 83:464-469. Tan EM. Antinuclear antibodies in diagnosis and management. Hosp Pract (Off Ed). 1983;18:79-84. Tan EM. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol. 1989;44:93-151. Kiviti S, Gilburd B, Agmon-Levin N et al. A novel automated indirect immunofluorescence autoantibody evaluation. Clin Rheumatol. 2011 Nov 5. [Epub ahead of print] Disclosure of Interest: None Declared