Phocaeicola faecicola sp. nov., isolated from porcine faeces. Issue 9 (21st September 2021)

• Record Type: Journal Article
• Authors: Choi, Ji Young; Choi, Seung-Hyeon; Park, Jam-Eon; Kim, Ji-Sun; Lee, Jiyoung; Lee, Mi-Kyung; Lee, Jung-Sook; Lee, Ju Huck; Jung, Hyunjung; Hur, Tai-Young; Kim, Hyeun Bum; Lee, Ju-Hoon; Kim, Jae-Kyung; Kang, Se Won; Park, Seung-Hwan
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 9(2021)
• Publication Date: 2021-09-21
• Subjects: Bacteroides -- Obligate anaerobe -- Phocaeicola faecicola -- Porcine faeces; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005008
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An obligately anaerobic, non-motile, Gram-negative and rod-shaped strain (AGMB03916 T ) was isolated from faeces of a 2-week-old piglet raised at the National Institute of Animal Science in Wanju, Republic of Korea. Growth of strain AGMB03916 T occurred at 30–45 °C (optimum, 37 °C), at pH 6–9 (optimum, pH 8) and in the presence of 0.5–1.0 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence analyses, strain AGMB03916 T was closely related to two validly published species of the genus Phocaeicola, Phocaeicola plebeius and Phocaeicola coprocola . The 16S rRNA gene sequence similarity of strain AGMB03916 T compared to P. plebeius M12 T (=KCTC 5793 T ) and P. coprocola M16 T (=KCTC 5443 T ) were 96.3 and 95.0 %, respectively. The genomic DNA G+C content of strain AGMB03916 T was 46.4 mol%. The average nucleotide identity values between strain AGMB03916 T and the reference strains were 74.9–78.5 %. Cells were able to utilize d -glucose, lactose, sucrose, maltose, salicin, aesculin hydrolysis, cellobiose and raffinose. The major end product of metabolism was acetate. The major cellular fatty acids (>10 %) of the isolate were iso-C15 : 0, anteiso-C15 : 0, C16 : 0, C16 : 0 3-OH and summed feature 11 (iso-C17 : 0 3-OH and/or C18 : 2 DMA). On the basis of the genotypic, biochemical, chemotaxonomic, phenotypic and phylogenetic data, strain AGMB03916 T represents a novel species of the genus Phocaeicola, for which the name Phocaeicola faecicola sp. nov. is proposed. The type strain is AGMB03916 T (=KCTC 25014 T =GDMCC 1.2574 T ).