THU0054 Interleukin-33/St2 Axis in Primary SjÖGren's Syndrome: Expression in Serum and Salivary Gland, and Clinical Association. (10th June 2014)

• Record Type: Journal Article
• Authors: Jung, S.M.; Kang, J.Y.; Min, H.K.; Koh, J.H.; Suh, Y.S.; Lee, J.H.; Lee, J.; Lee, J.Y.; Kim, J.-M.; Ju, J.H.; Park, K.-S.; Park, S.-H.; Kim, H.-Y.; Kwok, S.-K.
• Is Part Of: Annals of the rheumatic diseases. Volume 73:Supplement 2(2014)
• Page Start: 194
• Page End: 195
• Publication Date: 2014-06-10
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2014-eular.4921
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: IL-33 and its receptor, ST2, are associated with many inflammatory disorders. However, the data on IL-33/ST2 in Sjögren's syndrome (SS) is very limited. Objectives: To evaluate the expression of IL-33 and its receptor in sera and salivary tissues obtained from patients with primary Sjögren's syndrome (pSS) and to investigate the possible relationship with clinical profiles. Methods: Serum IL-33 and soluble ST2 (sST2) of 55 patients with pSS and 48 controls were determined using ELISA, and assessed for correlation with clinical profiles. The expression of IL-33/ST2 in salivary tissues was investigated by immunohistochemical (IHC) staining, and was further characterized by confocal microscopy. We also measured ST2 expression in peripheral blood mononuclear cells (PBMCs) of pSS patients, and IL-33 production in salivary glandular epithelial cell by pro-inflammatory stimuli. Results: The serum levels of IL-33 and sST2 were higher in pSS patients compared to healthy controls ( P =0.018 and P <0.0001, respectively). Among pSS patients, serum sST2 concentration was associated with thrombocytopenia ( P =0.029) and duration of disease ( p =0.013). Contrary to sST2, ST2 was downregulated in PBMCs obtained from pSS patients. The expression of IL-33 and ST2 was elevated in salivary gland of pSS patients with grade 2 inflammation, and decreased as inflammation progresses. In pSS patients, IL-33 was mainly observed in epithelial cells and endothelial cells of gland tissue. The production of IL-33 mRNA by salivary gland epithelial cell line was increased under stimulation with interferon-γ. Conclusions: IL-33 and sST2 concentrations were elevated in sera of pSS patients, whereas ST2 in salivary gland and PBMC of pSS patients showed downregulated expression. This result suggests that IL-33/ST2 axis might have a role in the pathogenesis of pSS. References: Schmitz, J., et al., IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity, 2005. 23(5): p. 479-90. Palmer, G. and C. Gabay, Interleukin-33 biology with potential insights into human diseases. Nat Rev Rheumatol, 2011. 7(6): p. 321-9. Moussion, C., N. Ortega, and J.P. Girard, The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel `'alarmin"? PLoS One, 2008. 3(10): p. e3331. Acknowledgements: This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI13C0016). Disclosure of Interest: : None declared DOI: 10.1136/annrheumdis-2014-eular.4921